Kangwon National University
Nanobio Engineering Lab
Kinetic stability modulation of polymeric nanoparticles for enhanced detection of influenza virus via penetration of viral fusion peptides
학술지 : Journal of Materials Chemistry B
논문 번호 : Volume 9, Number 47, 14 December 2021, Pages 9658-9669
Specific interactions between viruses and host cells provide essential insights into material science-based strategies to combat emerging viral diseases. pH-triggered viral fusion is ubiquitous to multiple viral families and is important for understanding the viral infection cycle. Inspired by this process, virus detection has been achieved using nanomaterials with host-mimetic membranes, enabling interactions with amphiphilic hemagglutinin fusion peptides of viruses. Most research has been on designing functional nanoparticles with fusogenic capability for virus detection, and there has been little exploitation of the kinetic stability to alter the ability of nanoparticles to interact with viral membranes and improve their sensing performance. In this study, a homogeneous fluorescent assay using self-assembled polymeric nanoparticles (PNPs) with tunable responsiveness to external stimuli is developed for rapid and straightforward detection of an activated influenza A virus. Dissociation of PNPs induced by virus insertion can be readily controlled by varying the fraction of hydrophilic segments in copolymers constituting PNPs, giving rise to fluorescence signals within 30 min and detection of various influenza viruses, including H9N2, CA04(H1N1), H4N6, and H6N8. Therefore, the designs demonstrated in this study propose underlying approaches for utilizing engineered PNPs through modulation of their kinetic stability for direct and sensitive identification of infectious viruses.
Active colorimetric lipid-coated polyaniline nanoparticles for redox state sensing in cancer cells
학술지 : Journal of Materials Chemistry B
논문 번호 : Volume 9, Number 14, 14 April 2021, Pages 3131-3135
Herein, lipid-coated polyaniline (LiPAni) nanoparticles were fabricated to monitor the redox state of cancer cells. To confirm the characteristics of LiPAni, we firstly analyzed the size and chemical structures of the LiPAni nanoparticles. The absorbance properties of the LiPAni nanoparticles were observed to vary with the pH conditions. Furthermore, cell viability tests conducted with breast cancer cell lines showed that the cell viability of the cells with LiPAni nanoparticles was dramatically increased compared to those with the Tween80-coated polyaniline nanoparticles (TPAni) as a control. Subsequently, the colors of the LiPAni nanoparticles were observed and analyzed using spectroscopic methods. Finally, in order to investigate the more accurate sensing of the redox state using the color changes of the LiPAni nanoparticles with cancer cell lines, dark field microscopic images and scattering spectra were recorded at the single nanoparticle scale. For the TPAni nanoparticles, there was only a change in brightness and no change in color, but for the LiPAni nanoparticles, there was a change of color from yellow to pink in the dark field images.
Point‐of‐Care Diagnostics: Host Cell Mimic Polymersomes for Rapid Detection of Highly Pathogenic Influenza Virus via a Viral Fusion and Cell Entry Mechanism
학술지 : Advanced Functional Materials
논문 번호 : Volume 28, 20 August 2018, 1800960
Since the highly pathogenic avian influenza virus (HPAIV) has crossed the species barrier to humans, early and accurate diagnosis between the highly pathogenic and the lowly pathogenic form of the virus is considered the key issue. FluSome, reported by Daesub Song, Seungjoo Haam, and their co‐workers in article 1800960, is a novel highly specific surveillance tool for carrying out early preventative procedures for controlling the HPAIV outbreaks.
Antiviral Agents: Reactive Oxygen Species‐Regulating Polymersome as an Antiviral Agent against Influenza Virus
학술지 : Small
논문 번호 : Volume13, August 25, 2017, 1700818
In article number 1700818, Daesub Song, Seungjoo Haam, and co‐workers, propose poly (aniline‐co‐pyrrole) polymerized nanoregulators (PASomes) with an amphiphilic methoxy polyethylene glycol‐block‐polyphenylalanine co‐polymer (mPEG‐b‐pPhe) to regulate intracellular ROS levels. PASomes are water‐soluble, biocompatible, and could suppress viral infection, thereby increasing the cell survival rate.
A Biodegradable Polymersome Containing Bcl‐xL siRNA and Doxorubicin as a Dual Delivery Vehicle for a Synergistic Anticancer Effect
학술지 : Macromolecular Bioscience
논문 번호 : Volume13, June 2013, Pages 745-754
Bcl‐xL siRNA and DOX encapsulated polymersomes (CPSomes) are synthesized using amphiphilic methoxy‐poly(ethylene glycol)‐block‐poly(lactic acid) copolymers to enhance the stability and delivery efficacy of their contents. Successful delivery of drugs and genes by CPSomes shows its potential as a dual delivery vehicle for cancer treatment. Further details can be found in the article by H.‐O. Kim, E. Kim, Y. An, J. Choi, E. Jang, E. B. Choi, A. Kukreja, M.‐H. Kim, B. Kang, D.‐J. Kim, J.‐S. Suh, Y.‐M. Huh,* and S. Haam* on page 745.